rat anti mouse cd205 mab  (Bio-Rad)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes.

doi: 10.4049/jimmunol.172.7.4091

Figure Lengend Snippet: FIGURE 2. The effect of anti-mouse SHPS-1 mAb or CD47-Fc treatment on the number of LCs in the epidermis after hapten application. Rat anti-mouse SHPS-1 mAb (B) or rat IgG (A and C) was intradermally injected before DNFB application. The ears of the mice were then painted with 10 l of 0.5% DNFB solution (A and B) or vehicle (C). Epidermal samples were collected and stained with PE-conjugated anti-mouse I-Ab mAb 24 h later. Bars 20 m. D and F, The density of I-Ab or CD11c cells in the epidermis is decreased in the rat IgG plus DNFB treatment group, whereas the anti-SHPS-1 mAb plus DNFB treatment group showed a lesser decrease in LC numbers. , p 0.01; , p 0.05; n 4. Error bars indicate SDs. E and G, Ears of mice were injected intradermally with 30 g of CD47-Fc fusion protein instead of anti-SHPS-1 mAb, and then DNFB was applied to the ears. The density of I-Ab or CD205 cells in the epidermis is decreased in the control human IgG-Fc treatment group, but not in the CD47-Fc treatment group (, p 0.01; , p 0.05; n 4). Error bars indicate SDs.

Article Snippet: Rat anti-mouse CD205 (DEC205) mAb was purchased from Serotec (Oxford, U.K.).

Techniques: Injection, Staining, Control